Christen Eriksen scored in the 11th minute to break a slump but it wasn't enough as Spurs fell to Chelsea 2-1 at Stamford Bridge.
The Danish Football Association confirm Christian Eriksen will not face the Czech Republic on Tuesday because of injury.
Christian Eriksen has signed a new four-year contract that will see the Dane play with Tottenham until 2020.
Real Madrid are looking to deal out playmaking midfielder Isco and reports indicate that Tottenham would be the likeliest destination for the Spanish international.
While various media sources have reported that Christian Eriksen is demanding a wage increase lest he puts in a transfer request, the Denmark international has spoke out against the inaccurate rumor.
Tottenham and Liverpool are both in pursuit of Bayern Munich's playmaking maestro Mario Gotze, with Spurs apparently in the pole position.
Christen Eriksen broke a 1-1 deadlock late in the match and Spurs made North London two for two in Showdown Sunday clashes as Tottenham beat Manchester City 2-1 at the Etihad.
After conceding the opening goal, Tottenham would score four times, two by Christian Eriksen, to topple Sunderland 4-1 at White Hart Lane.
Tottenham erased two deficits by scoring from two free kicks as Tottenham were able to grab a point against Swansea City, 2-2 at the Liberty Stadium.
Tottenham named the youngest XI in the league thus far this season, and the kids did well to handle a hot Crystal Palace side with a 1-0 victory.
Mauricio Pochettino has revealed that several key midfielders, including Christian Eriksen, will miss out on Tottenham's Europa group stage match tomorrow with Qarabag FC.
Christian Eriksen will miss out on Tottenham's Week 5 tilt with Sunderland because of his lingering knee issue, but Mauricio Pochettino made a point that the midfielder "is close".
Mauricio Pochettino has informed the media that Christian Eriksen is still out of action ahead of Tottenham's clash with Everton on Saturday.
Erik Lamela was handed his opportunity to shine in the place of the injured Christian Eriksen on Saturday, but once again the Argentine failed to deliver.